The presence of antibiotic-resistant bacteria is a disease that is reported to cause the most deaths in the world in the coming years. Less and fewer antibiotics are being produced to kill antibiotic-resistant bacteria. This is because the antibiotic spectrum is about to expire. On the other hand, after these resistant bacteria infect our body, high doses and more effective antibiotics are used to break this resistance gained by the bacteria. However, since these antibiotics affect and kill our healthy microflora, there is a possibility that they may have side effects on our bodies. Mycobacterium tuberculosis bacterium causes Tuberculosis (TB), one of the oldest known human diseases, and it is still one of the most important causes of death, causing two million deaths each year. The resistance of M. tuberculosis bacteria causes the tuberculosis disease to become more insoluble. This thesis aims to show that antibiotic-resistant Mycobacterium smegmatis can be non-resistant with the CRISPR / Cas method in an in-vitro laboratory environment.