Very cold regions are highly stressful environments because they affect biochemical reaction rates, substrate transport and properties, membrane mobility, and the structure of macromolecules such as DNA and proteins, and greatly alter the physicochemical environment of living cells. Organisms living in psychrophilic conditions adapt to low temperatures, and the folding ability of the proteins. The cpn60 and cpn10 chaperones have a positive effect on growth at low temperatures by increasing the refolding capacity of proteins at low temperatures. HSP60 (Heat shock protein-60), also known as chaperonins (Cpn), has important roles in the regulation and protection of both prokaryotic and eukaryotic cells, especially during environmental stress. Here we discuss that it has been discussed that the bacteria in the genus Acetobacter can be compared with each other by phylogenetic analysis, determining which species are more resistant to cold. After the phylogenetic analysis, it will be discussed that variants in the Acetobacter genus that express a heat-shock protein or are resistant to heat-shock stress can be taken. Here we determine the most efficient temperature value by culturing at various temperatures.