The HIV (Human Immunodeficiency Virus) virus can settle in a variety of human tissues and is spread by blood and unprotected sexual contact, but it mostly affects the immune system. Today's anti-HIV medications stop the virus's growth in the body and its immunosuppressive effects, enabling HIV-positive persons to live a longer, healthier life. Early treatment initiation and consistent treatment maintenance under a doctor's supervision are crucial for this. In the treatment and progression of HIV infection, early diagnosis and, consequently, early treatment are important. In addition to increasing life expectancy, early diagnosis also lowers transmission rates. Today, RT-PCR and antibody-based diagnostic kits are used for HIV diagnosis. But both diagnostic models have some flaws and glitches. To eliminate this situation, CRISPR-based diagnostic kits are very suitable for use in the diagnosis of both viral and bacterial diseases.